Analysis of Alternative Splicing and Alternative Polyadenylation in Populus alba var. pyramidalis by Single-Molecular Long-Read Sequencing

Analysis of Alternative Splicing and Alternative Polyadenylation in Populus alba var. pyramidalis by Single-Molecular Long-Read Sequencing

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Doormat Poplars are worldwidely cultivated with ecologically and economically important value.Populus alba var.pyramidalis (= P.bolleana) is a main tree of the farmland shelter-belt system in the arid region of Northwest China due to its rapid growth, erect stems, and high biomass production.

However, the full-length messenger RNA (mRNA) sequences and complete structure of P.alba var.pyramidalis remain unclear.In this study, using single-molecular real-time (SMRT) and next-generation high-throughput sequencing (NGS) platform, we sequenced transcripts from leaf, root, xylem, and phloem of P.

alba var.pyramidalis, to obtain the full-length mRNA transcripts and annotate the complete structure.In total, 86,327 mapped full-length non-chimeric (FLNC) reads were identified, with 705 previously unannotated loci and 3,410 long noncoding RNAs (lncRNAs) and 174 fusion genes found.Alternative spicing (AS) events were detected in 7,536 genes, Course a pied - Femme - Vetements - Pantalon - Nylon of which 4,652 genes had multiple AS events.

A total of 10,213 alternative polyadenylation (APA) sites were identified, with two or more APA sites observed in 2,212 genes.Our transcriptome data provided the full-length sequences and gene isoforms of transcripts for P.alba var.pyramidalis, which will be helpful in improving our understanding for the genome annotation and gene structures of P.

alba var.pyramidalis.